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KMID : 0613820190290060688
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Journal of Life Science
2019 Volume.29 No. 6 p.688 ~ p.696
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Effect of Fermented Ice Plant Extract on the Inhibition of Triglyceride and Cholesterol Synthesis and Tyrosinase Activity
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Nam Sang-Hae
Kim Seon-Jeong
Ko Keon-Hee
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Abstract
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This study investigated changes in triglyceride and cholesterol synthesis and tyrosinase activity induced by ice plant (Mesembryanthemum crystallinum L.) extract, which cannot be stored for long periods of time due to its high moisture content when it was fermented to improve its storage stability. The accumulation of triglyceride and cholesterol in HepG2 cells inhibited the accumulation with a relatively large magnitude in n-butanol and aqueous fractions that generally have high polarity, however, changes in inhibition potency due to the fermentation were not significant. As for the effect to inhibit tyrosinase activity, when L-tyrosine was used as a substrate, the inhibitory activity was the highest for the aqueous fraction at 60.58±4.03% and 63.35±4.35%, before and after fermentation, respectively, which amounted to 72% of that of the positive control group (arbutin, 100 μg/ml). In addition, when L-3,4-dihydroxyphenylalanine (L-DOPA) was used as a substrate, the inhibitory activity was also found the highest for the aqueous fraction at 56.85±1.57% and 59.38±1.74%, before and after fermentation, respectively, which amounted to at least 88% of that in the positive control (kojic acid, 100 μg/ml). Overall, the activity of the fermented ice plant extract was similar or a little higher compared to that of the one without fermentation, indicating that fermentation can be a good approach to improve the storage stability of the ice plant.
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KEYWORD
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Cholesterol synthesis, cyclitol compounds, Ice plant (Mesembryanthemum crystallinum L.), triglycerides, tyrosinase activity
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